Cell Culture and Fermentation Summer Intern

We are seeking sophomore and junior students majoring inChemical Engineering, Bioengineering, Biology, Statistics, Computer Science,or a related field for six 12-Week Student Intern roles in Gaithersburg, MD for a May 19th - August 8th contract period.

Position Duties:

There are six separate headcount for the descriptions below.

Cell Culture Intern:

• Plan and execute laboratory studies using design of experiments.

• Run small-scale production bioreactors and evaluate culture performance.

• Perform analytical tests and interpret data using statistical analysis.

• Present progress at department meetings and prepare a final poster presentation.

Fermentation Intern:

• Perform CRISPR-Cas9/Recombineering9,10 to knock out thyA in attenuated E. coli expression strains.

• Confirm gene knockout via polymerase chain reaction (PCR), next-generation sequencing (NGS), and western immunoblotting.

• Clone an in-house antibody fragment (GP130 and/or ALXN1720) into an expression plasmid.

• Transform and express GP130 and/or ALXN1720 in thymidine auxotrophs and compare productivity against unmodified E. coli.

Tech transfer digitalization and manufacturing data management:

• Develop tools to improve tech transfer process workflows and gain efficiency for process knowledge transfer.

• Improve ways of working by automating manufacturing data collection and visualization for use by cell culture development and tech transfer teams.

• Generate new templates and test new functionalities to support digitization of tech transfer processes.

Position Requirements:

• Undergraduate sophomore and junior students majoring inChemical Engineering, Bioengineering, Biology, Statistics, Computer Science,or a related field.

• Candidates must have an expected graduation date after August 2025.

• Coding skills such as Matlab and/or Python are required.

• Wet lab skills such as cell cultures, pipetting, sterile technique are a plus.

• Experience with PowerBI, Excel, and SharePoint are a plus.

• Enthusiasm for collaboration, cross-functional projects, public speaking, and presentation design.

• US Work Authorization is required at time of application.

• This role will not be providing CPT/OPT support.

• Ability to report onsite to Gaithersburg, MD for 3-5 days a week. This role will not provide relocation assistance.

• Minimum grade point average of 3.0